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Korean J Spine > Volume 2(2); 2005 > Article
2
Induction of Annular Repair by the Implantation of Cultured Annulus Cell
Jae-Taek Hong, M.D.1, Il-Woo Lee, M.D.3, Sang-Won Lee, M.D.1, Byung-Chul Son, M.D.1, Hyeon-Ju Choi, M.D.2, Min-Woo Baik, M.D.4, Moon-Chan Kim, M.D.5
Department of Neurosurgery1 and Pathology2, St. Vincent Hospital, The Catholic University of Korea, Suwon, Korea, Department of Neurosurgery, Daejon St. Mary's Hospital, The Catholic University of Korea, Daejon, Korea3, Department of Neurosurgery, Holy Fa
배양된 섬유륜 조직 세포의 이식을 통한 섬유륜 손상의 재생 유도
홍재택,이일우,이상원,손병철,최현주,백민우,김문찬
가톨릭대학교 의과대학 신경외과학교실
Abstract
Objective
s: The aims of this study were to assess the regeneration capability of intervertebral disc after annulotomy using tissue engineering techniques.

Methods
Tissue engineering, cell culture, microdiscectomy, histological test, and immunohistochemical staining method were applied. The annulus fibrosus tissue was obtained from the intervertebral tissue of inbred Fisher rats, cultured, incised the annulus fibrosus of another same strain white rat by microdiscectomy, cultured annulus fibrosus cells were transplanted by using a scaffold. In the same animal, one area was treated only with the incision of the annulus fibrosus and another area was transplanted only a scaffold after the incision of the annulus fibrosus, and these areas served as control groups for the degenerative change and the proliferation of transplanted cells. The regenerative capacity of transplanted cells was assessed by histological test and immunohistochemical staining.

Results
The results were that in the group transplanted cultured annulus fibrosus tissue with a scaffold, the increased proli- feration of intervertebral disc cells and the reduction of the degenerative change were observed, and regenerated annulus fibrosus tissue was similar to the normal annulus fibrosus histologically.

Conclusion
The data suggest the possibility of applying the transplant of the annulus fibrosus using a scaffold for the regeneration of intervertebral disc and the prevention of the degenerative change, and, in addition, it suggests the feasibility of the effective utilization of the model of the degenerative change of the intervertebral disc of Fischer rat used in this study to similar cell transplant experiments in future.
Keywords: Intervertebral disc.Regeneration.Annulus fibrosus.Cell culture.Tissue engineering


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