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Proteoglycan Dysfunction as a Key Hallmark of Intervertebral Disc Degeneration: Commentary on “Proteoglycan Dysfunction: A Common Link Between Intervertebral Disc Degeneration and Skeletal Dysplasia”

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Neurospine. 2024;21(1):179-181
Publication date (electronic) : 2024 March 31
doi : https://doi.org/10.14245/ns.2448266.133
Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
Corresponding Author Takashi Yurube Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan Email: takayuru-0215@umin.ac.jp

Low back pain is a global health problem with a markedly high lifetime prevalence— 70%–85% [1]—and socioeconomic burden—up to $102.0 billion/yr in the United States [2], which is also the most common reason for the worker’s disability [3]. Although the cause of low back pain is largely nonspecific, a large-scale twin study has found intervertebral disc degeneration as the independent, main risk factor for disabling low back pain [4]. A population study of magnetic resonance imaging has further identified the increase in the prevalence of lumbar disc degeneration with age, based on 42% of 18–30 years and 88% of 50–55 years in age [5], thereby facilitating impaired daily activities of the elderly [6]. Along with severe low back pain, intervertebral disc degeneration can cause neurological disorders such as radiculopathy, myelopathy, paralysis, intermittent claudication, and even bladder and bowel dysfunction [7]. Despite successful conservative treatment for degenerative disc disease [6], nonresponders need surgery [7]. The current primary surgical approach is symptomatic disc excision and/or spinal fusion, which results in the loss of load bearing, shock absorption, and movement [7]. Therefore, the development of new biological therapies for degenerative disc disease is an urgent demand to restore the physiological function.

The intervertebral disc is unique, as the largest immune-privileged, low-nutrient, avascular organ in the human body [8]. The disc has a complex structure of the central nucleus pulposus (NP) encapsulated by the peripheral annulus fibrosus (AF) and sandwiching cartilage endplates [7]. The collagenous, laminar AF maintains the pressurization of the gelatinous, oval NP, which provides support during compressive loading and multidimensional spinal movement [9]. In the disc development, while the AF comes from the mesenchyme [10], the NP originates from the notochord [11]. In human life, disc NP notochordal cells only exist during the first 10 years [10], later replaced by nonnotochordal, chondrocyte-like cells arising from NP cells as a terminal differentiation stage during degeneration [12]. The phenotype of AF cells is thus chondrocytic while NP cells have the chondrocytic or notochordal phenotype depending on the age and degeneration severity [13]. Accompanied with the notochordal phenotype disappearance and chondrocyte proliferation, cell death increases between 11–16 years of age in the disc [14]. Under stress [15], cells can fall into the irreversible growth arrest of senescence, producing proinflammatory cytokines and matrix-degrading proteases through the senescence-associated secretory phenotype [16]. Excessive stress ultimately leads to programmed cell death including apoptosis, necroptosis, pyroptosis, and ferroptosis [16]. Autophagy, the intracellular degradation and recycling system, also plays a role in maintaining cell homeostasis [16,17]. Taken together, cell fate with the phenotypic changes should be involved significantly in the pathogenesis of intervertebral disc degeneration.

Morphological disc degeneration also begins during early childhood [14,18], which is more severe in the NP than in the AF [14]. Disc NP clefts and radial tears can appear between 11–16 years of age [14]. Biochemically, disc cells have anabolic activities through the production of extracellular matrix components, e.g., proteoglycans—primarily aggrecan—and collagens—principally types I in the AF and II in the NP [7]. Aggrecan biosynthesis and procollagen type-II content peak at ≤ 5 years and diminish between 5–15 years of age [18]. Meanwhile, denatured collagen type II increases after the age of 5 years [18]. This matrix degradation is regulated by the balance between catabolic enzymes, e.g., matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs), and anticatabolic proteins, e.g., tissue inhibitors of metalloproteinases (TIMPs) [19]. In fact, increased MMP and ADAMTS activity relative to TIMP expression has been shown in human [20,21] and rodent experimental disc degeneration [22]. Hence, extracellular matrix degradation is a key hallmark of intervertebral disc degeneration.

A narrative literature review article by Sao and Risbud [23], provides the extensive, expertized summary of proteoglycans, a major component of the extracellular matrix. Proteoglycans consist of a core protein covalently attached with glycosaminoglycan chains. Proteoglycans serve for the hydration absorbing and retaining water in the tissue through their sulfated glycosaminoglycans, which are essential for the disc to support the trunk. Proteoglycans are categorized into the 4 groups based on the type of glycosaminoglycan chains: chondroitin sulfate/dermatan sulfate, heparan sulfate, keratan sulfate, and hyaluronic acid [24]. These four types are further categorized depending on the size of proteoglycans: large and small [24]. In the extracellular matrix, while large proteoglycans provide water-binding properties, tissue hydration, and growth factor bioavailability, small proteoglycans regulate collagen fibril formation [24]. Recently, the categorization based on the location has been suggested: extracellular, pericellular, cell surface, and intracellular [24]. Aggrecan is the most abundant, large extracellular proteoglycan with chondroitin sulfate/dermatan sulfate chains. Versican is another large extracellular proteoglycan with chondroitin sulfate chains. Lubricin has been shown as an atypical extracellular proteoglycan. Biglycan and decorin are small extracellular proteoglycans with chondroitin sulfate/dermatan sulfate chains. Fibromodulin and tenomodulin are also small proteoglycans present in the extracellular matrix, contributing to the tissue structural integrity. Syndecans, particularly syndecan-4, are a cell surface proteoglycan, which mediate multiple musculoskeletal diseases through inflammation, such as rheumatoid arthritis, osteoarthritis, and intervertebral disc degeneration [25]. Perlecan is a large pericellular proteoglycan with heparan sulfate/chondroitin sulfate chains. This review article [23] describes in detail not only intervertebral disc and cartilage pathologies but also skeletal dysplasias in humans and mouse models resulting from the loss or reduction of proteoglycans. Serious congenital abnormalities and embryonic lethality by the deletion of proteoglycans indicate their essentiality in skeletal development and integrity. In particular, the structure and function of major proteoglycans should be known, as spine surgeons, specialists, and researchers often manage discs and facet joints. Finally, as a conclusive remark [23], the authors further propose potential biological therapies to treat disc degeneration and restore the matrix function, including senolytic approaches. This paper [23], published in the March 2024 issue of the Neurospine, provides the fundamental basis and important updates for a better understanding of intervertebral disc cell matrix biology.


Conflict of Interest

The author has nothing to disclose.


1. Andersson GB. Epidemiological features of chronic low-back pain. Lancet 1999;354:581–5.
2. Martin BI, Deyo RA, Mirza SK, et al. Expenditures and health status among adults with back and neck problems. JAMA 2008;299:656–64.
3. Guo HR, Tanaka S, Halperin WE, et al. Back pain prevalence in US industry and estimates of lost workdays. Am J Public Health 1999;89:1029–35.
4. Livshits G, Popham M, Malkin I, et al. Lumbar disc degeneration and genetic factors are the main risk factors for low back pain in women: the UK Twin Spine Study. Ann Rheum Dis 2011;70:1740–5.
5. Cheung KM, Karppinen J, Chan D, et al. Prevalence and pattern of lumbar magnetic resonance imaging changes in a population study of one thousand forty-three individuals. Spine (Phila Pa 1976) 2009;34:934–40.
6. Yurube T, Ito M, Takeoka T, et al. Possible improvement of the sagittal spinopelvic alignment and balance through “locomotion training” exercises in patients with “locomotive syndrome”: a literature review. Adv Orthop 2019;2019:6496901.
7. Urban JP, Roberts S. Degeneration of the intervertebral disc. Arthritis Res Ther 2003;5:120–30.
8. Takeoka Y, Yurube T, Nishida K. Gene therapy approach for intervertebral disc degeneration: an update. Neurospine 2020;17:3–14.
9. Hartman RA, Yurube T, Ngo K, et al. Biological responses to flexion/extension in spinal segments ex-vivo. J Orthop Res 2015;33:1255–64.
10. Hunter CJ, Matyas JR, Duncan NA. The notochordal cell in the nucleus pulposus: a review in the context of tissue engineering. Tissue Eng 2003;9:667–77.
11. Choi KS, Cohn MJ, Harfe BD. Identification of nucleus pulposus precursor cells and notochordal remnants in the mouse: implications for disk degeneration and chordoma formation. Dev Dyn 2008;237:3953–8.
12. Mohanty S, Pinelli R, Pricop P, et al. Chondrocyte-like nested cells in the aged intervertebral disc are late-stage nucleus pulposus cells. Aging Cell 2019;18:e13006.
13. Poiraudeau S, Monteiro I, Anract P, et al. Phenotypic characteristics of rabbit intervertebral disc cells. Comparison with cartilage cells from the same animals. Spine (Phila Pa 1976) 1999;24:837–44.
14. Boos N, Weissbach S, Rohrbach H, et al. Classification of age-related changes in lumbar intervertebral discs: 2002 Volvo Award in basic science. Spine (Phila Pa 1976) 2002;27:2631–44.
15. Risbud MV, Shapiro IM. Role of cytokines in intervertebral disc degeneration: pain and disc content. Nat Rev Rheumatol 2014;10:44–56.
16. Yurube T, Takeoka Y, Kanda Y, et al. Intervertebral disc cell fate during aging and degeneration: apoptosis, senescence, and autophagy. N Am Spine Soc J 2023;14:100210.
17. Yurube T, Ito M, Kakiuchi Y, et al. Autophagy and mTOR signaling during intervertebral disc aging and degeneration. JOR Spine 2020;3:e1082.
18. Antoniou J, Steffen T, Nelson F, et al. The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration. J Clin Invest 1996;98:996–1003.
19. Vo NV, Hartman RA, Yurube T, et al. Expression and regulation of metalloproteinases and their inhibitors in intervertebral disc aging and degeneration. Spine J 2013;13:331–41.
20. Roberts S, Caterson B, Menage J, et al. Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc. Spine (Phila Pa 1976) 2000;25:3005–13.
21. Pockert AJ, Richardson SM, Le Maitre CL, et al. Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degeneration. Arthritis Rheum 2009;60:482–91.
22. Yurube T, Takada T, Suzuki T, et al. Rat tail static compression model mimics extracellular matrix metabolic imbalances of matrix metalloproteinases, aggrecanases, and tissue inhibitors of metalloproteinases in intervertebral disc degeneration. Arthritis Res Ther 2012;14:R51.
23. Sao K, Risbud MV. Proteoglycan dysfunction: a common link between intervertebral disc degeneration and skeletal dysplasia. Neurospine 2024;21:162–78.
24. Iozzo RV, Schaefer L. Proteoglycan form and function: a comprehensive nomenclature of proteoglycans. Matrix Biol 2015;42:11–55.
25. Wang J, Markova D, Anderson DG, et al. TNF-alpha and IL-1beta promote a disintegrin-like and metalloprotease with thrombospondin type I motif-5-mediated aggrecan degradation through syndecan-4 in intervertebral disc. J Biol Chem 2011;286:39738–49.

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